Research on the cognitive neural mechanism of privacy empowerment illusion cues regarding comprehensibility and interpretability for privacy disclosures.

In the era of artificial intelligence, privacy empowerment illusion has become a crucial means for digital enterprises and platforms to "manipulate" users and create an illusion of control. This topic has also become an urgent and pressing concern for current research. However, the existing studies are limited in terms of their perspectives and methodologies, making it challenging to fully explain why users express concerns about privacy empowerment illusion but repeatedly disclose their personal information. This study combines the associative-propositional evaluation model (APE) and cognitive load theory, using event-related potential (ERP) technology to investigate the underlying mechanisms of how the comprehensibility and interpretability of privacy empowerment illusion cues affect users' immediate attitudes and privacy disclosure behaviours; these mechanisms are mediated by psychological processing and cognitive load differences. Behavioural research results indicate that in the context of privacy empowerment illusion cues with low comprehensibility, users are more inclined to disclose their private information when faced with high interpretability than they are when faced with low interpretability. EEG results show that in the context of privacy empowerment illusion cues with low comprehensibility, high interpretability induces greater P2 amplitudes than does low interpretability; low interpretability induces greater N2 amplitudes than does high interpretability. This study extends the scopes of the APE model and cognitive load theory in the field of privacy research, providing new insights into privacy attitudes. Doing so offers a valuable framework through which digital enterprises can gain a deeper understanding of users' genuine privacy attitudes and immediate reactions under privacy empowerment illusion situations. This understanding can help increase user privacy protection and improve their overall online experience, making it highly relevant and beneficial.

Multi-omics profiling reveal responses of three major Dendrobium species from different growth years to medicinal components.

Dendrobium is a perennial herb found in Asia that is known for its medicinal and ornamental properties. Studies have shown that the stem is the primary medicinal component of Dendrobium spp. To investigate the effect of the species and age of Dendrobium (in years) on the content of its medicinal components, we collected the stems of 1-to-4-year-old D. officinale, D. moniliforme, and D. huoshanense, sequenced the transcriptome, metabolome, and microbiome, and analyzed the data in a comprehensive multi-omics study. We identified 10,426 differentially expressed genes (DEGs) with 644 differentially accumulated metabolites (DAMs) from 12 comparative groups and mapped the flavonoid pathway based on DEGs and DAMs. Transcriptomic and metabolomic data indicated a general trend of the accumulation of flavonoids exhibiting pharmacological effects in the three Dendrobium species. In addition, joint metabolome and microbiome analyses showed that actinobacteria was closely associated with flavonoid synthesis with increasing age. Our findings provide novel insights into the interactions of flavonoids of Dendrobium with the transcriptome and microbiome.

Comprehensive Microbiome and Metabolome Analyses Reveal the Medicinal Components of Paeonia lactiflora.

Paeonia lactiflora Pall. is not only a traditional ornamental plant, but also an important medicinal plant. Currently, some P. lactiflora cultivars are used for ornamental purposes, but their potential medicinal value is ignored. To explore the medicinal potential of the ornamental varieties, the medicinal cultivar 'Hangbaishao' (HS) and the ornamental cultivar 'Zifengyu' (ZFY) were selected, and microbiome and metabolome analyses were performed to compare the composition of the endophytes and metabolites in the roots. The diversity and abundance of bacteria were not significantly different between HS and ZFY; however, the diversity and abundance of endophytic fungi in the ornamental cultivar ZFY were much higher than those in the medicinal cultivar HS. The flavonoids and phenolic acid contents of the ornamental cultivar ZFY were significantly higher than those of the medicinal cultivar HS, indicating that ZFY has medicinal value. The differences in root endophytes between HS and ZFY may lead to differences in phenolic acids and flavonoids. To explore the relationship between endophytes and the accumulation of phenolic acids and flavonoids, a joint analyses of the microbiome and metabolome were performed. The key bacterium, Ruminococcaceae bacterium GD7, led to the accumulation of phenolic acids and flavonoids in the ZFY. This study contributes to future research on the potential medicinal value of ornamental P. lactiflora and provides a new approach for realizing the 'dual use of medicine and appreciation' of P. lactiflora.

A New Explanation for the Attitude-Behavior Inconsistency Based on the Contextualized Attitude.

Inconsistency between attitude and behavior is a major obstacle to research on the predictive power of attitudes on behavior. To clarify the mechanism underlying such inconsistency, we combined event-related potential (ERP) and questionnaires to explore the relationship between contextualized attitudes and retrospective attitudes in the context of illusion of privacy empowerment (IPE). When the participants read the IPE events (including platform empowerment intention, technique, result, etc.) on slides, we measured retrospective attitudes with questionnaires and recorded contextualized attitudes with ERPs. We found that individuals' retrospective attitudes were different from contextualized attitudes: retrospective attitudes were mainly affected by the individual's analytic system, while contextualized attitudes were mainly affected by the direct stimulus-response (i.e., heuristic system). Therefore, retrospective attitudes may not accurately reflect individual cognition in the immediate context, and inconsistency between attitudes and behavior may be caused by the mismatch between retrospective attitudes and immediate behavior. Our findings provide a more reasonable account of the relationship between attitudes and behavior.

Soil properties, microbial diversity, and changes in the functionality of saline-alkali soil are driven by microplastics.

With the intensification of microplastic (MP) pollution, the impact of MPs on soil ecosystems has garnered considerable attention. We investigated the effects of two commonly used MPs, polyethylene (PE) and polypropylene (PP), at different sizes and doses, on the properties and microbial communities in saline-alkali soil. We found that MP treatment significantly reduced the electrical conductivity but somewhat enhanced the enzyme activities and effective nutrient content of the soil. Microbial diversity is affected by the type, dose, size and interaction of MPs, with fungi being more sensitive than bacteria. Under high-dose PE treatment, the dominant bacteria and fungi enriched, and the diversity indexes declined significantly. Meanwhile, under high-dose PP treatment, several unique bacteria and fungi with low abundance were observed, which eventually increased the diversity indexes. Moreover, PE exerted a stronger effect on bacterial function than PP. High-dose PE treatment suppressed the nitrogen fixation potential of soil bacteria. However, high-dose PP treatment promoted that. In conclusion, our findings showed that PE exerts a stronger negative effect on saline-alkali soil ecosystems than PP. Our findings help bridge the knowledge gap in the impact of MPs on saline-alkaline soils and provide guidance for the rational use of agricultural plastics in saline-alkaline soils.

Analysis of niche shift and potential suitable distributions of Dendrobium under the impact of global climate change.

Dendrobium is a valuable traditional Chinese herb that contains active ingredients such as polysaccharides and alkaloids that have anti-aging, antioxidant, and immunomodulating effects. The appropriate distribution range of Dendrobium should be predicted from the perspective of ecological niche theory in order to preserve and utilize medicinal plant resources. In this study, Dendrobium nobile, Dendrobium officinale, and Dendrobium moniliforme were selected to predict the potential suitable distributions and ecological niche shifts. A comparison of 19 environmental variables of the three Dendrobium species revealed three climatic factors that differed significantly when the species were compared two at a time. The principal component analysis was carried out in order to screen seven climatic factors for ecological niche shift analysis. All three Dendrobium species were found to have a very similar ecological niche, but with a relatively small range of variability regarding certain climatic factors. Finally, the current and future suitable areas for these three Dendrobium species in China were predicted using the MaxEnt model and ArcGIS using the two representative concentration pathways (RCP 2.6 and 8.5). Overall, the analysis of the climatic factors' comparisons, niche shift, and current and future suitable areas of these three Dendrobium species provides a basis for medicinal plant resource conservation and utilization, and our methods could be applied to the study of other similar valuable medicinal plants.

Chaetomium globosum D5 confers salinity tolerance on Paeonia lactiflora Pall.

Plants will interact with beneficial endophytic fungi to increase resistance under environmental stress. Among these stresses, salt stress poses one of the major threats to plant growth worldwide. We have studied the response mechanism of Chaetomium globosum D5, a salt-tolerant fungus isolated from the roots of Paeonia lactiflora under salt stress, and its mechanism of action in helping P. lactiflora alleviate salt stress. In our study, high levels of salt inhibit growth, whereas low levels promote the growth of C. globosum D5, which resists salt stress by forming dense hyphae and producing more pigments, soluble proteins, and antioxidants. Under salt stress, growth and photosynthesis of P. lactiflora are inhibited, and they are subjected to osmotic stress, oxidative stress, and ionic stress. C. globosum D5 could help P. lactiflora promote growth and photosynthesis by increasing the uptake of nitrogen and phosphorus and increasing the accumulation of the carbon and photosynthetic pigments, help P. lactiflora alleviate osmotic stress by increasing the accumulation of proline, help P. lactiflora alleviate ion stress by reducing Na+ and increasing K+/Na+, Ca2+/Na+ and Mg2+/Na + ratios in P. lactiflora roots and leaves. In summary, joint action between P. lactiflora and C. globosum D5 is responsible for mitigating damage caused by P. lactiflora under salt stress. We first investigate the interaction between the fungus and P. lactiflora under salt stress, providing a theoretical basis for further investigations into the mechanisms of P. lactiflora's response to salt stress and its promotion in coastal areas.

Analysis of the different growth years accumulation of flavonoids in Dendrobium moniliforme (L.) Sw. by the integration of metabolomic and transcriptomic approaches.

Dendrobium moniliforme (L.) Sw. is a valuable herbal crop, and flavonoids are primarily distributed as active ingredients in the stem, but the composition and synthesis mechanisms of flavonoids in different growth years are not clear. The accumulation of flavonoids in D. moniliforme from four different years was investigated, using a combined metabolomics and transcriptomics approach in this study. The phenylpropanoid and flavonoid biosynthetic pathways were significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs). The widely targeted metabolomics technique revealed a total of 173 kinds of flavonoid metabolites. The metabolomics data confirmed the trend of total flavonoids (TF) content in stems of D. moniliforme, with chalcone, naringenin, eriodictyol, dihydroquercetin, and other flavonoids considerably up-accumulating in the third year. Twenty DEGs were detected that regulate flavonoid synthesis and the expression of these genes in different growth years was verified using real-time quantitative PCR (qRT-PCR). Furthermore, a comprehensive regulatory network was built for flavonoid biosynthesis and it was discovered that there is one FLS gene, one CCR gene and two MYB transcription factors (TFs) with a high connection with flavonoid biosynthesis by weighted gene co-expression network analysis (WGCNA). In this study, the correlation between genes involved in flavonoid biosynthesis and metabolites was revealed, and a new regulatory mechanism related to flavonoid biosynthesis in D. moniliforme was proposed. These results provide an important reference for the farmers involved in the cultivation of D. moniliforme.

What will polyethylene film mulching bring to the root-associated microbial community of Paeonia ostii?

Plastic film mulching can increase crop yield and is widely used in agricultural production, but long-term mulching could adversely affect plant growth. To investigate the related mechanism, we studied the bacterial communities in different root-associated compartments of Paeonia ostii, a perennial oil crop, under polyethylene film mulching for three years by full-length 16S rDNA sequencing technology, and measured the soil physicochemical properties and enzyme activities. We found that enzyme activities and available nutrients in the soil tended to decrease after long-term mulching. Analysis of bacterial community composition revealed that the endosphere may be another potential source of the root-associated microbiome of P. ostii, and the rhizoplane plays a selective gating role in the enrichment processes for P. ostii microbiome assembly. Long-term mulching affected the abundance of dominant bacterial communities in different root-associated compartments and reduced the bacterial richness in the endosphere, but increased bacterial interactions in each compartment, as well as between different compartments. We speculate that this is mainly related to the decrease of litter content and the serious degradation of polyethylene film after long-term mulching, which resulted in microplastics and other harmful substances entering the soil. Our study further explained the reasons for the harm of long-term film mulching on plants to guide the rational use of plastic film. KEY POINTS: •Soil enzyme activities and available nutrients decreased after long-term mulching. •Mulching affected the dominant bacterial abundance in different root-associated compartments. •Mulching increased bacterial interactions among compartments.

Integration of Transcriptome and Metabolome Provides New Insights to Flavonoids Biosynthesis in Dendrobium huoshanense.

Dendrobium huoshanense is both a traditional herbal medicine and a plant of high ornamental and medicinal value. We used transcriptomics and metabolomics to investigate the effects of growth year on the secondary metabolites of D. huoshanense stems obtained from four different years of cultivation. In this study, a total of 428 differentially accumulated metabolites (DAMs) and 1802 differentially expressed genes (DEGs) were identified. The KEGG enrichment analysis of DEGs and DAMs revealed significant differences in "Flavonoid biosynthesis", "Phenylpropanoid biosynthesis" and "Flavone and flavonol biosynthesis". We summarize the biosynthesis pathway of flavonoids in D. huoshanense, providing new insights into the biosynthesis and regulation mechanisms of flavonoids in D. huoshanense. Additionally, we identified two candidate genes, FLS (LOC110107557) and F3'H (LOC110095936), which are highly involved in flavonoid biosynthesis pathway, by WGCNA analysis. The aim of this study is to investigate the effects of growth year on secondarily metabolites in the plant and provide a theoretical basis for determining a reasonable harvesting period for D. huoshanense.

Transcriptome and metabolome profiling unveil the accumulation of flavonoids in Dendrobium officinale.

Dendrobium officinale is a Chinese herbal medicine with a long history of use in China. Flavonoids are known to be an important secondary metabolite in Dendrobium officinale, but very little is known about their molecular regulation mechanism in D. officinale. In this study, we collected one to four years old D. officinale stems for the purpose of RNA-sequencing and mass spectrometry data collection. The results showed that metabolome analysis detected 124 different flavonoid metabolites of which flavonol metabolites were significantly increased in biennial samples. In the transcriptome analysis, 30 different genes involved in the synthesis of flavonoid were identified. The key genes FLS (LOC110101392, LOC110107557, LOC110114894) that regulate the synthesis of flavonols are highly expressed in biennial samples. The present study contributes a new insight into the molecular mechanism of flavonoid accumulation in D. officinale.

Composition and diversity of bacterial communities in the rhizosphere of the Chinese medicinal herb Dendrobium.

BACKGROUND: Dendrobium is a precious herbal that belongs to Orchidaceae and is widely used as health care traditional Chinese medicine in Asia. Although orchids are mycorrhizal plants, most research still focuses on endophytes, and there is still large amount unknown about rhizosphere microorganisms. To investigate the rhizosphere microbial community of different Dendrobium species during the maturity stage, we used high-throughput sequencing to analyze microbial community in rhizosphere soil during the maturity stage of three kinds of Dendrobium species. RESULTS: In our study, a total of 240,320 sequences and 11,179 OTUs were obtained from these three Dendrobium species. According to the analysis of OTU annotation results, different Dendrobium rhizosphere soil bacteria include 2 kingdoms, 63 phyla, 72 classes, 159 orders, 309 families, 850 genera and 663 species. Among all sequences, the dominant bacterial phyla (relative abundance > 1%) were Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, Chloroflexi, and Gemmatimonadetes. And through WGCNA analysis, we found the hub flora was also belong to Acidobacteria, Actinobacteria and Proteobacteria. CONCLUSIONS: We found that the rhizosphere bacterial communities of the three kinds of Dendrobium have significant differences, and that the main species of rhizosphere microorganisms of Dendrobium are concentrated in the Proteobacteria, Actinobacteria, and Bacteroidetes. Moreover, the smaller the bacterial level, the greater the difference among Dendrobium species. These results fill knowledge gaps in the rhizosphere microbial community of Dendrobium and provide a theoretical basis for the subsequent mining of microbial functions and the study of biological fertilizers.